Archive for » 十一月, 2013 «

星期五, 十一月 29th, 2013 | Author:

Nearly half (46%) of lung cancer patients report experiencing delays at some stage of their care – and only two thirds (64%) say they receive prompt referral to hospital – according to a national report published today (28 November) by leading lung cancer experts.

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星期五, 十一月 29th, 2013 | Author:

IntroductionEpithelial-to-mesenchymal transition (EMT) promotes cell migration and is important in metastasis. Cellular proliferation is often downregulated during EMT and the reverse transition (MET) in metastases appears to be required for restoration of proliferation in secondary tumors. We studied the interplay between EMT and proliferation control by MYB in breast cancer cells.
Methods:
MYB, ZEB1 and CDH1 expression levels were manipulated by lentiviral small hairpin RNA (shRNA)-mediated knockdown/overexpression, and verified by Western blotting, immunocytochemistry and qRT-PCR. Proliferation was assessed by bromodeoxyuridine pulse labeling and flow cytometry, and sulforhodamine B assays. EMT was induced with epidermal growth factor for 9 days or by exposure to hypoxia (1% oxygen) for up to 5 days, and assessed by qRT-PCR, cell morphology and colony morphology. Protein expression in human breast cancers was assessed by immunohistochemistry. ZEB1-MYB promoter binding and repression were determined by Chromatin Immunoprecipitation Assay and a luciferase reporter assay, respectively. Student’s paired T tests, Mann–Whitney and repeated measures 2-way ANOVA tests determined statistical significance (P < 0.05).
Results:
Parental PMC42-ET cells displayed higher expression of ZEB1 and lower expression of MYB than the PMC42-LA epithelial variant. Knockdown of ZEB1 in PMC42-ET and MDA-MB-231 cells caused increased expression of MYB and a transition to a more epithelial phenotype, which in PMC42-ET cells was coupled with increased proliferation. Indeed, we observed an inverse relationship between MYB and ZEB1 expression in two in vitro EMT cell models, in matched human breast tumors and lymph node metastases, and in human breast cancer cell lines. Knockdown of MYB in PMC42-LA cells (MYBsh-LA) led to morphological changes and protein expression consistent with an EMT. ZEB1 expression was raised in MYBsh-LA cells and significantly repressed in MYB over-expressing MDA-MB-231 cells, which also showed reduced random migration and a shift from mesenchymal to epithelial colony morphology in two dimensional monolayer cultures. Finally, we detected binding of ZEB1 to MYB promoter in PMC42-ET cells, and ZEB1 over-expression repressed MYB promoter activity.
Conclusions:
This work identifies ZEB1 as a transcriptional repressor of MYB and suggests a reciprocal MYB-ZEB1 repressive relationship, providing a mechanism through which proliferation and the epithelial phenotype may be coordinately modulated in breast cancer cells.

星期五, 十一月 29th, 2013 | Author:

Researchers at the UNC School of Medicine, working with cell lines in a lab, have discovered why some of the most aggressive and fatal breast cancer cells are resistant to chemotherapy, and UNC scientists are developing ways to overcome such resistance.Adriana S.

星期五, 十一月 29th, 2013 | Author:

Breast cancer will likely affect one in eight women at some point during their lifetime, and the risks increase with age. However, the risk is not the same for all women in any given age group.

星期五, 十一月 29th, 2013 | Author:
For the first time ever, the H6N1 bird flu has turned up in a human being, a 20-year-old Taiwanese woman who was hospitalized earlier this year after she developed a high fever, cough and shortness of breath. According to the Associated Press (AP), the woman somehow…
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星期五, 十一月 29th, 2013 | Author:
Adding vitamins to your diet can help reduce the feeling of depression, winter blues or seasonal affective disorder (SAD). B vitamins, especially vitamin B12, have been shown to help those suffering from feelings of anxiety or depression. Simply adding nutritional supplements…
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星期五, 十一月 29th, 2013 | Author:
Four out of five people regularly stop breathing while typing emails, according to studies conducted by former Apple executive Linda Stone. The condition, which health professionals are calling “email apnea,” may lead to serious health consequences. ”If people are…
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星期五, 十一月 29th, 2013 | Author:
Cancer, chronic fatigue, diabetes, osteoporosis plus many other degenerative diseases are caused by ‘metabolic acidosis.’ Is your body pH slightly alkaline? If not, this is a serious health condition – that needs to be addressed immediately. Shocking ‘sick care’ statistics…
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星期五, 十一月 29th, 2013 | Author:
She went from the administration’s hero to zero with the click of a mouse. Once touted by the White House as an Obamacare success story, Jessica Sanford has now discovered that, no, she won’t be able to afford new health insurance coverage, thanks to the very law…
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星期五, 十一月 29th, 2013 | Author:
Striking on any plate, squid ink-infused cuisine is much more than a pretty edible – new research indicates that these unconventional foods are brimming with health advantages. Whether arroz negro of Spain or Venetian fettuccine al nero di seppia, the dark color of these…
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